Product Placement

As shown in this diagram, using the needle method, filler is typically injected into the low dermis (corium) and subcutaneous level of skin. The dermis layer of the skin is between the epidermis and subcutaneous tissues. The subcutaneous tissue, which is also known as hypodermis, is the innermost layer of skin. It is made up of fat and connective tissues that contain larger blood vessels and nerves.

The epidermis of thick skin consists of five layers: stratum basale, stratum spinosum, stratum granulosum, stratum lucidum and stratum corneum.

With injection of the Hyaluron Pen, it is the epidermis layers of skin that we are mainly dealing with (as shown in diagram). The product is injected with a spray pattern releasing the product into the epidermis region. This spray technique is what gives the enhancement a very smooth and even appearance as it distributes evenly.  

Hyaluron Pen Injection vs. Needle Injection

The spray-pattern of the Hyaluron Pen is demonstrated above. Because of this spray-like technique, the time it takes for Hyaluronic Acid gel from the Hyaluron Pen method to spread is only 3-7 days. In contrast, the needle injection method can take up 20 days which is why lumps left behind at injection site are more likely.

What is Vascular Occlusion? 

 Vascular Occlusion is from accidental needle injection of filler into an artery or when too much filler is deposited, causing the arteries to compress or collapse. This is a big advantage of pen enhancements because most vessels are located much lower in the skin to where pen enhancements can reach. The HA gel molecules are squeezed into a microscopic size and then spread out into a fan type of injection when they enter the skin rather than being deposited into a larger ball or large stream.Vascular occlusion is so rare, even with needle injections, but if it does happen, it can be addressed rather quickly by seeking medical attention from a physician who would then inject a medicine called hyaluronidase into the area completely dissolving all the filler in that area.


The diagrams below offer more information on the main differences of the product placements.  

Facial Rejuvenation Procedure  

As we all know there are numerous ways we can rejuvenate the facial skin using topical serums, different types of technology and machines, but you can also rejuvenate the entire face with injectable serums using the Hyaluron Pen. 

By performing .01 injections, spaced out about 3/4 to 1” apart using Hyaluronic Acid, you can not only increase fullness and firmness to mature or sagging skin, but also increase your patient/ client’s natural production of collagen by introducing Hyaluronic acid into the epidermis. 

This same technique can be used with other beneficial gel-like serums like mesotherapy serums with .01 or .02 injections. 

This is one of the great things that the Hyaluron Pen can do that your standard needle injection cannot. With its ability to spray the filler in rather than straight lines of injection, you cover larger areas with a single injection, and the filler begins to travel within that zone producing more collagen.    

Use of the Hyaluron Pen & Pen Placement

In this lesson we are going to go over the body of the Hyaluron Pen. We will discuss how the pen works, the design and the ampoules we use to inject the product. We will also discuss the additional items needed to complete your procedure from start to finish. Make sure to take notes as we proceed with the visual demonstration.

• Hyaluron Pen 

• Ampoules 

• Hyaluronic Acid Syringe 

• Needle Tip for Filling Ampoule 

• Loading Ampoule 

• Prepping The Area For Injection 

• Pen Placement 

• Dispensing Product 

• Removing Ampoule 

• Sterilizing your Pen    

How To Fill Your Ampoules & Loading Your Hyaluron Pen

You will find that most hyaluronic acid gels or fillers will come in a separate syringe. This means that you will need to fill your ampoules, properly transferring the product from the syringe to the ampoule. Purchasing dispensing tips to attach to the syringe will help the product enter the ampoule with ease and less air bubbles. Again, we want to remind you that it is important to always purchase your ampoules in the corresponding amount to your hyaluron pen (eg. .5ml ampoules for .5ml pen, .3ml ampoules for a .3ml pen). Once you have opened your hyaluronic acid, it is important to keep it refrigerated. In most cases, it will be good for one year unopened on the shelf or six months once opened and refrigeration but please check with your Hyaluronic Acid brand manufacturer to be sure of their storing instructions. The following videos will show you how to properly fill your ampoules, load them into your hyaluron pen, and store them after being filled. 

Use of Products & Table Setup

These are the proper materials that you should have included your table setup. Next to each one is a brief description why they are necessary. 


Table Towel- this will essentially be your placemat used to keep your items sanitary

Metal Tray- this is what you will set majority of your items on, placed on top of your table towel

Hyaluron Pen- either your .5ml or .3ml hyaluron pen

Hyaluronic Acid Filled Ampoules- make sure these are prefilled prior to your client arriving. Have them ready on your table setup when they arrive. If they were placed in cold refrigeration, take them out 20 minutes prior. 

Sharps Disposal Container- have this for disposing your ampoules when needing to change them out and any other items that may need disposing of. 

Alcohol Wipes- for proper sanitation and sterilization of both your materials and the injection area

4” Cotton Squares- to wipe areas and to also aid your grip inside the lip region

Hand Mirror- for the client to be able to see their results along the way and provide feedback on their desired results

Protective Face Mask- important for protecting both yourself and client

Practitioner Gloves- again, this is important for sanitary reasons for both you and your client

Aesthetic Q-Tip- to apply your aftercare solutions 

Petroleum Jelly- can be applied after injections to calm the area 

Optional- numbing cream If your client is sensitive to pain but there should be relatively no pain involved


Disclaimer of Medical and Legal Liability: Aesthetics Accreditation International training courses are intended to provide the general knowledge to perform procedures but is not intended to be a substitute for medical advice, diagnosis, or treatment. Reliance on the information in this training course for procedural purposes is to be used at your own risk. If you have questions or concerns, contact a medical professional prior to treatment. AAI is not held responsible or liable for risks involved with this procedure. 

AAI strongly advises each member or student to research their local legislation. It is your sole responsibility to check and clarify all rules and regulations pertaining to your country, state, city, and county if you are planning on performing our training program procedures as a professional. AAI is not held responsible to provide this information and AAI cannot guarantee this information for any person. Please check with your local health department, governing boards, and FDA regulations regarding performance of any AAI course procedure. AAI is not held responsible or liable for legal encounters regarding licensing, regulations, or other legal aspects pertaining to procedural operation.